Nucleus purification/isolation protocols?

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Research about using a orange peel pectin derived agar in cultivating bacteria that is very versatile?

It is about my research using a orange peel pectin derived agar in cultivating bacteria that is very versatile and can grow on wide variety of conditions, can you suggest me a gram positive...

09 May 2024 7,376 0 View

Why does my bone slice of a mouse femur have this huge gap between the bone marrow and the cortex of the bone?

A picture of a bone slice of mouse femur with bone marrow being slightly red.

14 February 2024 7,714 4 View

Classification tables in logistic regression?

Would anyone happen to know how the percentages are calculated in SPSS for the predicted and observed categories? Is it something you can do by hand or does SPSS use some kind of internal...

08 January 2024 252 4 View

Is it possible to check that an antibody is successfully attaching to its antigen via mass spectrometry?

I am looking for a simple way to determine if antibody fragments (fabs) are able to attach to their target after they have been fragmented. I was wondering if it is possible to simply mix the...

10 December 2023 9,441 0 View

Why is there DNA in my mRNA sample after in vitro transcription?

I use the MEGAscript T7 Transcription Kit (Cat. no.: AMB13345, ThermoFisher) for in vitro transcription of linearized plasmids. However, I have measured a high concentration of DNA in my mRNA...

26 November 2023 2,931 3 View

What is the best measurement of organizational trust?

I want to measure the level of organizational trust for a leadership study. Which would you recommend?

14 November 2023 513 5 View

What are the best ways to measure stress - biometric or otherwise - for a leadership study?

Hi all, I am thinking of collecting data on stress for a leadership study I am doing. For HRV, I was thinking to use wearable devices. But I am also thinking of cortisol strips. Any other good...

17 September 2023 4,969 3 View

Why pseudo second order kinetics model work for physisorption ?

Hello, We work on CO2 adsorption on active carbon and we find experimentally that this adsorption is physisorption. Next we try to fit the pseudo-first order that should work for physisorption...

01 June 2023 3,861 0 View

How does sampling efficiency in droplet-based assays affect sensitivity?

For droplet-based assays, such as digital-droplet PCR or similar, it is common to image/process only a selection of the generated droplets. Conceptually, I would think that increasing the number...

25 May 2023 1,967 1 View

Why the Thomas model work and the LDF doesn't ?

Hello, I'm working on two matematic's models : the LDF model and the Thomas model. We modelised breakthough curves (the concentration as a function of the time) and we find that the Thomas model...

22 May 2023 1,146 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Can i apply a public questionnaire but analyse it with a different data analysis method?

First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

27 July 2024 3,457 3 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

Do we put respondents data in research methods or findings section of a qualitative study?

Are both ways acceptable, or is there any convention to them? Thanks.

22 July 2024 6,988 4 View

Restaurant research, methods of quantitative and qualitative statistics?

Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...

20 July 2024 2,033 7 View