Hello everyone,
I have used Anti-Nrf2 Antibody from Proteintech( 16396-1-AP, 1:1000 in 5% milk, 1.5hr at RT) in F344 rat kidney nuclear extract and I found a strong band at around 130KD along with non-specific bands at around 70 and 25KD, whereas in HK-2 cell lysates, the band was at 110KD. I also used Nrf2 siRNA and sulforaphane treated HK2 samples and I found the difference in band intensity at 110KD. I am now confused about the 130KD band in rat samples. Attached is the image of the blot.
Can anyone kindly suggest the possible reason for this difference in sizes of Nrf2 in rats and HK2 samples?