Razia Sultana Mohammad

5 Questions 13 Answers 0 Followers

Questions related from Razia Sultana Mohammad

Mitochondrial complex II+IV coupled assay?

Hello everyone, I did a complex II+III coupled assay following the procedure described by AntoniBarrientos, in "In vivo and in organello assessment of OXPHOS activities". In vivo and in...

30 September 2020 998 3 View

Inverted peaks in the Imagelab lane profile?

Hello everyone, I have TIFF images of western blots that were acquired using Syngene chemidoc machine. I have converted TIFF files into 8-bit images using ImageJ (as there was a error opening the...

15 September 2020 5,230 5 View

Nrf2 band at 130KD in nuclear extract of rat kidney?

Hello everyone, I have used Anti-Nrf2 Antibody from Proteintech( 16396-1-AP, 1:1000 in 5% milk, 1.5hr at RT) in F344 rat kidney nuclear extract and I found a strong band at around 130KD along with...

28 January 2020 6,904 0 View

Has anyone tried preparing Dimethyl fumarate in 0.08% Methyl celluose by sonication?

Hello everyone, I wanted to make 100mg/ml of Dimethyl fumarate(DMF) in 0.08% methyl cellulose(MC). Its not miscible in MC, so tried sonicating on ice. My question is, can we store the sonicated...

24 May 2019 1,678 1 View

GFP tagging using CRISPR/Cas9 approach: how many guide RNAs will help in getting specific knockin?

Hii everyone, I want to tag GFP to a gene of interest (GOI) at N-terminal domain rather than at C-terminal using CRISPR-Cas 9 system. I have designed a gRNA which targets a region 200 bp upstream...

25 May 2016 3,706 10 View