what is normale? do you mean normal and resistant prostate cancer cells?

I mean a prostate cell line which is not derived from a prostate tumor but from 'normal' prostate tissue, probably immortalized, like PNT2C2 or BPH-1.

http://www.atcc.org/Portals/1/Pdf/CellCatalog/CellTissue.pdf

Spontaneously immortalized adult non-tumorigenic human prostate epithelial cell lines: NHPrE1 and BHPrE1 from Simon Hayward's lab at Vanderbilt.

I am using PZ-HPV 7 as prostate normal cells.

PNT2 cells from SIGMA are normal prostate epithelium immortalized with SV40 cells and are luminal (CK-18 and CK-8). Does anyone knows if normal basal prostate cell lines are commercially or even not commercially available? They should express exclusively CK-5 and CK-14 but not CK-18 or CK-8 markers. Thank you!

normal prostate cell? 

RWPE-1

Helloo every one, my question to all of you is tht , what can be the alternative media for growing RWPE1 CELL LINE.some papers have used RPMI1640. Kindly reply with your experiances.

NHPrE1 and BHPrE1 cell line were established in our lab.

I have found that RWPE-1 is anything but normal; with its expression of E6/E7 oncoproteins, the cell line appeared to display fairly cancer-like characteristics when comparing against PNT1a, PNT2 and the classic PCa cell lines. We are using PWR1E as a 'normal' cell simply because it expresses AR vs the other non-malignants, though I have not compared this against the others.