Hey, i'm looking for a 'normale' prostate cell line. Has someone an idea if there is such a cell line??
Thanks, Hetty
what is normale? do you mean normal and resistant prostate cancer cells?
I mean a prostate cell line which is not derived from a prostate tumor but from 'normal' prostate tissue, probably immortalized, like PNT2C2 or BPH-1.
Spontaneously immortalized adult non-tumorigenic human prostate epithelial cell lines: NHPrE1 and BHPrE1 from Simon Hayward's lab at Vanderbilt.
PNT2 cells from SIGMA are normal prostate epithelium immortalized with SV40 cells and are luminal (CK-18 and CK-8). Does anyone knows if normal basal prostate cell lines are commercially or even not commercially available? They should express exclusively CK-5 and CK-14 but not CK-18 or CK-8 markers. Thank you!
Helloo every one, my question to all of you is tht , what can be the alternative media for growing RWPE1 CELL LINE.some papers have used RPMI1640. Kindly reply with your experiances.
I have found that RWPE-1 is anything but normal; with its expression of E6/E7 oncoproteins, the cell line appeared to display fairly cancer-like characteristics when comparing against PNT1a, PNT2 and the classic PCa cell lines. We are using PWR1E as a 'normal' cell simply because it expresses AR vs the other non-malignants, though I have not compared this against the others.
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