No flurecence at one PCR well?

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Ahmed AbdulJabbar Suleiman

May be the DNA destroyed in this period or your primers contam you can check the DNA by use another primer

Fatma Abdel Wahab

Dear Ahmed The Sample in the same run had also , other 4 genes screening

They all give Amplfication plot

so you can screen about mutation in the primer annealing region

Can it be two samples ?

Does this finding go with Sample evaopration?