After drying my DNA samples for 20mins under a Bunsen at room temperature I can't see any pellet and no DNA shows up on a gel, despite having pellet in the previous step (precipitation, washing etc). Any thoughts about what I could be doing wrong? My process is precipitate at -80 over the weekend, centrifuge 14000g 10mins to give pellet, wash 75% ethanol before air dry and resuspension.
Since you did not provide the details of your protocol, it would not be possible to suggest any reason about what could go wrong.
DNA extraction is not very hard method and if you are not getting anything in the end, there is something you are doing wrong. Find any good working protocol and compare it with yours. You will find some mistake, or you will be getting something in next isolation.
if you electrophoresed the product and you didn't get DNA bands. I think that the pellets you noticed were not DNA. maybe protein or....while the DNA may not be seen
in the suspended solution.
Repeat extraction precisely...
- Badges
- Science topic
- Similar topics
- Geoscience
- Atmospheric Sciences
- Atmosphere
- Air