Cleaning up single cell RNA from 10x dataset?

Hello, I am new to single cell RNA data processing and am currently running my data through the initial cleanup steps via Seurat on R. I have previously done this on epithelial organoid data and...

23 December 2020 4,709 1 View

Fluorimeter giving odd results for carbon quantum dots?

I made a Pb sensor using carbon quantum dots (CQDs). I add buffer, CQDs, and a given metal to a quartz cuvette of the recommended type. Before adding a metal, the cuvette will emit a yellow-green...

16 December 2020 9,675 3 View

Recommend resources for developing surveys of technology-users for "needs-analysis" or design criteria?

I am looking for recommendations for good resources or guidelines for developing surveys specifically focused on targeting user's opinions or a needs-analysis in the context of engineering design...

12 December 2020 6,698 6 View

How much salt in a m^3 of water would be needed to prevent evaporation ?

Assume you have 1 cubic meter of water with 1 square meter of surface exposed to the sun. If you kept adding salt to the solution, is there a point at which evaporation would cease? Assuming the...

22 November 2020 5,444 1 View

No DNA pellet after air drying?

After drying my DNA samples for 20mins under a Bunsen at room temperature I can't see any pellet and no DNA shows up on a gel, despite having pellet in the previous step (precipitation, washing...

08 November 2020 7,775 2 View

Automatic cell counting? (Ductal epithelium cells with fluorescence)?

Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...

20 September 2020 1,855 6 View

Resuspension mutant huntington (mHTT) in conditioned medium?

Hello all. In lab we grow cells to secret mHTT in media that we then collect and subject to centrigufation at 100k G for 2 hours to concentrate the protein and other secreted components of the...

30 August 2020 1,858 1 View

How to improve my CBD to THC conversion method?

Hello Everyone, Let me preface this question by saying that this is all being done under a legal THC/Hemp license in the state of Colorado. I was hoping to get some advice on how to improve my...

27 April 2020 6,292 1 View

Is there literature on pre-steady state "lag" for oxidase enzymes?

Greetings, I'm looking for literature related to a topic I want to write a manuscript on. I'm working with an glycine oxidase enzyme and noticed when I monitor the rate via spectrophotometry and a...

31 March 2020 3,917 2 View

What's going on with my HPLC?

Hi Everyone, I was wondering if you guys had any ideas/suggestions that I can work with to try to fix my HPLC. I have attached a picture of one of our Blanks (100% ACN). This shape is unusual,...

16 March 2020 6,454 5 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View