I would like to measure the amount of LRP1 receptor on cell surface of different cell types, I am struggling to find a ICC protocol or method?

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Can two biological replicates in a 7 day repeated measurement, suffice for statistics?

Experimental set-up: I have recorded plant performance values in triplicate on two plants from a 4x3x2 factorial designed experiment, over 7 days As I have only two biological replicates per...

12 October 2022 5,498 3 View

No DNA pellet after air drying?

After drying my DNA samples for 20mins under a Bunsen at room temperature I can't see any pellet and no DNA shows up on a gel, despite having pellet in the previous step (precipitation, washing...

08 November 2020 7,957 2 View

How to culture of keratinocytes from human skin punch biopsy?

Hi, I have been having problems culturing keratinocytes from 4mm human skin punch biopsies. When I recieve the biopsy I first was the samples in sterile PBS, then I remove the subcutaneous fat. I...

24 April 2020 2,962 2 View

What are the possible indels generated when NHEJ is activated via CRISPR Cas9?

Hi, So I've got the bi-allelic sequence of several Knock Out cell lines I've generated. I assume that the mutations have been caused by NHEJ after the DS break by Cas9. There is variation in the...

02 February 2020 2,361 2 View

RNAi for nuclear transcripts?

Is it possible to do RNA interference for RNA knock down on non coding RNA transcripts that are only present in the nucleus of human cells?

24 March 2019 7,711 3 View

FastQC for RNA-Seq-Fails for per base seq content, gc content, duplication levels, adapter content and kmer content?

Hi, Currently i am performing QC on new RNA-seq data. My fastq files were generated by Paired-end sequencing with a read length of 151 bases. Total rna was extracted and depleted of rRNA. RNA was...

13 February 2019 541 1 View

How to equip a lab for plant pathogen research?

Hi all, I’m working on a project for developing models for disease progression in plants, early diagnostics, and treatment recommendations using sensor arrays and AI. Additionally we’re looking...

11 February 2019 6,550 1 View

Can anyone please recommend an antibody for the detection of Fra 1 & 2 (fos related antigen) in rat brain?

Can anyone please recommend an antibody for the detection of Fra 1 & 2 (fos related antigen) in rat brain? We have been using sc-253 successfully but this antibody is now...

29 January 2017 4,208 0 View

Does anyone know of a repository for spectral libraries of oil/slick types?

Similar to the USGS spectral library for materials, but for marine slicks. http://speclab.cr.usgs.gov/spectral-lib.html

31 March 2016 8,586 3 View

Anyone familiar with InSAR deformation vs SAR DEM?

Can someone please explain to me how InSAR is capable of such high accuracy deformation studies (millimetre accuracy) but SAR DEMs are nowhere near as accurate? Is it down to the resolution? Is...

27 July 2015 8,087 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View