Hello,
I am currently working on my Master Thesis, and I need to grow fresh-water Oscillatoria sancta in the lab. I have read from multiple sources that nitrogen-fixing filamentous cyanobacteria needs to grow on nitrogen-free medium. I have tried looking for nitrogen-free BG11 medium, however, I can only find it at UTEX's website, and I'm not sure if they ship the medium to Europe.
Therefore, I would like to ask if there is an alternative to nitrogen-free BG11 medium?
Thank you in advance!
Hi Maria, making BG-11 yourself isn't all that hard if you have the appropriate chemicals and an autoclave to sterilize the media. See attached for our recipe.
Dear Maria,
Hereby, please find two recent publications about cyanobacteria, which may help you find what you want to know about its methodology.
The short communication published in 2021 is a good one to refer to its references and gives a good idea of overall researches around Cyanobacteria.
The paper published in 2019 may give you tips in methodology of Cyanobactetia grown in harsh environmental situations.
Good luck.
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