Looking for a protocol for Nissl stain for fresh frozen, not fixed mouse brain. Any preferences for Cresyl violet over thionine? Thanks!
Hello! I'm having trouble removing alcian blue specks from my zebrafish larvae stains. See photo for specks of alcian blue in the right corner of ANC. I'm following the Walker & Kimmel...
16 August 2023 9,208 3 View
This is a centric species with coarse areolae and two thick processes on opposite sides of the valve. Is it a kind of Aulacoseira? or Actinocyclus? Doesn't seem to fit the definitions for either...
14 August 2023 6,539 4 View
Can you implant an optic fiber for transient illumination without having to leave the cannula implanted? We're trying to setup a photothrombotic stroke model in the mouse hippocampus and need to...
10 May 2021 8,797 3 View
Hi! I'm looking to assess the extent of kidney damage/fibrosis with MTS and some of my tissue has a very weird coloring. I'm not sure if this expected from injured tissue or is a stain artifact....
28 May 2020 9,977 0 View
I've a significant positive direct effect between the DV and IV. When I add the interaction term (moderator) - the effect disappears... it's not longer significant. Also the effect of the...
27 April 2019 3,834 30 View
Hi! I'm looking for an easy/inexpensive way to measure renal function in mice. So far have found colorimetric ELISA kits but there some concern with accuracy on mice samples. Any advice? Thanks!
17 June 2018 1,108 3 View
Hi there, I am extracting RNA from environmental samples like soil. I have a three stage protocol. First I use a Chloroform/Phenol based extraction according to Toewe et all, then I am using a...
13 March 2017 779 7 View
I direct a program of studies on neurocognition, substance abuse and HIV. Our previous recruitment strategies (HIV or SUD treatment facilities, community organizations and word of mouth) work...
24 June 2015 7,675 15 View
I've been using Naphrax for some time now, but compared to Hyrax (which has been long gone - and is still sorely missed), I find that slides made with Naphrax do not have the same high-quality...
19 June 2014 2,336 9 View
I isolated an Algibacter lectus from my plant tissue, however Algibacter lectus is capable to degrade agar, gelatine and starch. So to confirm that he degrades a specific carbon source, which I...
30 January 2013 9,601 28 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...
01 August 2024 9,903 7 View