I would like to know the simple invitro assay protocol for testing our recombinant antibody against Staphylococcus and Pseudomonas strains for their neutralizing capacity using above strains. (how much bacterial inoculum required?, )
Thanks. I will serially dilute antibody and add equal quantity of 0.5 McFarland inoculum in it and incubate at 37oC for 2 hrs. Then add in it growth medium. Control will be inoculum in growth medium, and only growth medium. Next day check OD600. Is it OK. Is there any reference protocol?