Can I use Neuroblastoma SH-SY5Y as a reference for the expression of neuronal markers ( Nestin - B III tubulin - GFAP - MAP2) to confirm the neuronal differentiation by real-time quantitative PCR.
Yes!!! There are so many papers in relation to it. Differentiated SH-SY5Y cells represents neurons so you easily detect above markers except GFAP(astrocytic marker).