Hi Michael,

It's a  bit complex since I don't know what kind of experiment you do. but there is a way to do negative selection...

Try this method. I used it when I isolated alveaolar type 2 cells from mouse but I did not do the negative selection of ECs

- Incubate  cells with the beads and with anti-PECAM-1 Abs. since the endothelial cells express PECAM-1 constitutively, the beads with the anti-bodies, should ideally go and bind to the cells of interest, from the cell suspension.

-the endothelial cell attached to the magnetic beads can be  recovered using a magnet.

- collect the rest of the supension  which do not contain the ECs with a plastic pipette

Best wishes!! 

Thanks Celimene

I use adherent HUVEC.  CD62 transiently expressed before re-internalizing .

Ab selection will not because trypsin will interfere. 

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