Célimène Galiger

4 Questions 21 Answers 0 Followers

Questions related from Célimène Galiger

Can anyone recommend good antibodies for pAkt Thr308, ALK, pALK Tyr1604 pBIM Ser69 TERT and PTERT Ser824 for western blot?

Hi everyone, does anyone has already tested good working pAkt-Thr308, ALK, pALK-Tyr1604 pBIM-Ser69 TERT and PTERT-Ser824 antibodies for westen blot on human cell lines? If yes, would you be kind...

19 August 2019 6,855 3 View

How can I improve PP2A activity in Neuroblastoma treated with OP449, an activator of PP2A phosphatase?

I would like to know if anybody is familiar with phosphatase activity assay and more precisely with PP2A activity in cells treated with OP449. First of all, I have already shown that neuroblastoma...

10 October 2018 1,127 2 View

How to improve phosphatase activity of PP2A using the PP2A immunoprecipitaion phosphatase assay kit from Millipore??

I would like to measure the activity of PP2A in neuroblastoma. So far I have tried many experiments and I am not able to get a very good activity. I am using the PP2A immunoprecipitation...

24 August 2018 2,885 5 View

How can I estimate the concentrations of packaging plasmids pxPAX2 and pMD2.G for lentivirus production using pTRIPZ as transgene?

Hi, I ordered pTRIPZ from Dharmacon and I would like to produce lentiviral particules. In their protocol, they recommend to use 42 ug of the pTRIPZ for each 100 mm culture plate containing 5.5...

19 April 2017 9,234 4 View