I am looking for the DNA sequence of a "run away plasmid" origin of replication that comes from a plasmid called pKN402. The Ori is in the family of replicons called IncFII or pT181 that represent an incompatibility group.
What I want to do is build a new plasmid using this Ori so I can mix it with other plasmids from other incompatibility groups.
This is my first post to ResearchGate which I joined just recently. Not sure if I am posting this question to the right group or a wide enough group.
Feedback welcomed, as well as the DNA sequence.
maybe you can have a try here:
http://bccm.belspo.be/db/lmbp_plasmid_details.php?NM=pCP3
...they say the parental plasmid is pKN402 ...so the ori should be similar of the same!
I also used pSC101 ori ...can be easily obtained from different sources.
Good luck!
Thank you for this tip. I had found this site last night, but it does not provide DNA sequence information. I am looking to have this Ori synthesized as part of a new plasmid I want to have built.
I am looking for an Ori that is not in either the green or the red incompatibility groups shown on this page.
http://www.bio.davidson.edu/courses/Molbio/Protocols/ORIs.html
Thanks for your help.
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