Can anyone tell me the steps of JoinMap 3.0 for making a genetic map along with the precautions I should take?

I am having SNP data of RIL populations and the Phenotypic data. Now I want to construct the linkage map using JoinMap. As I have no expertise of join map it would be nice if someone could provide...

11 December 2014 5,013 8 View

How long is it possible to store cDNA at -40c?

For how much time cDNA can be stored at -40 deg. Is it possible to store cDNA for nearly 6 months or above? Thanks for advise in advance

06 July 2014 7,156 21 View

Anyone have suggestions for separating SSR on Metaphor Agarose?

I am trying to separate the SSR markers of Venturia Inaequalis (Apple Scab Fungus) having Bp range of 171 t0 180 (maximum difference of 1bp to 7 bp). But, while separating using Metaphor Agarose,...

31 December 2013 4,568 9 View

Can anyone suggest how to do standardization of EST SSR's?

I designed SSR markers using MISA tool from the EST database downloaded from NCBI. The product size of one of the markers which I amplified in the PCR shows no specific binding. I also did...

10 November 2013 4,645 1 View

Primerpro tool?

We are trying to do Esr SSR analysis from a FASTA file using primer pro, it works nicely for "example file included in documentation " but when applied to our own multifasta file it shows some...

08 September 2013 8,908 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View