Need of Agrobacterium AGL-1 strain with pBHt2 vector for Colletotrichum lindemuthianum transformation?

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Can anyone tell me the steps of JoinMap 3.0 for making a genetic map along with the precautions I should take?

I am having SNP data of RIL populations and the Phenotypic data. Now I want to construct the linkage map using JoinMap. As I have no expertise of join map it would be nice if someone could provide...

11 December 2014 5,146 8 View

How long is it possible to store cDNA at -40c?

For how much time cDNA can be stored at -40 deg. Is it possible to store cDNA for nearly 6 months or above? Thanks for advise in advance

06 July 2014 7,405 21 View

Anyone have suggestions for separating SSR on Metaphor Agarose?

I am trying to separate the SSR markers of Venturia Inaequalis (Apple Scab Fungus) having Bp range of 171 t0 180 (maximum difference of 1bp to 7 bp). But, while separating using Metaphor Agarose,...

31 December 2013 4,750 9 View

Can anyone suggest how to do standardization of EST SSR's?

I designed SSR markers using MISA tool from the EST database downloaded from NCBI. The product size of one of the markers which I amplified in the PCR shows no specific binding. I also did...

10 November 2013 4,765 1 View

Primerpro tool?

We are trying to do Esr SSR analysis from a FASTA file using primer pro, it works nicely for "example file included in documentation " but when applied to our own multifasta file it shows some...

08 September 2013 9,024 1 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to back transform the results generated from analyses using log transformed with In(X+1) data?

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...

31 July 2024 7,860 3 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View