Rabia Arjamand

6 Questions 1 Answers 0 Followers

Questions related from Rabia Arjamand

Which transport medium is best for E.coli?

I'm working on Microbiology EQA program. I have confirmed E.coli strains in my lab. Now I want to transport them to other labs at a distance of 10-12 hrs. we don't want to use ice-packs during...

24 July 2024 1,810 0 View

Can we use DH5-alpha for conjugation assays (mcr gene transmission)?

I'm working on HGT of colistin resistance in E.coli. Is In most of the papers, E.coli J53 is used, I don't have that strain , can I use DH5-alpha for this purpose. Normally E.coli J53 has sodium...

01 February 2024 886 0 View

How to troubleshoot Easyfig?

I'm working on comparative genomics. I've to compare plasmids in terms of genes. In most of the papers, I've read about Easyfig. Initially, I faced trouble with blast downloading, later I...

21 January 2024 1,801 1 View

Need E.coli J53 for conjugation assays, where to get it from Pakistan?

I'm performing conjugation assays in E.coli. I need E.coli J53 as recipient strain. This strain is not available in my university. If anyone have this strain in their lab kindly tell me. thankyou

14 January 2024 7,508 0 View

Which tools should be used for comparative analysis of mcr bearing plasmids isolated from different sources?

I'm working on HGT of colistin resistance through plasmids. I've done plasmid typing and mapping. I've samples from different poultry sources. To perfrom comparative genomics, I started learning...

14 January 2024 7,092 1 View

Finding E.coli J53 for conjugation assays

I've to perform conjugation assays for my research experiment. I'm working on plasmid mediated colistin resistance in E.coli. For that purpose, I require E.colo J53, sodium azide resistant strain...

01 January 1970 7,305 0 View