Straightly, two assay on male mice & female mice to induce EAE were carried out by me.
Here is the statistics:
Male:
WT: 4/6 incidence, mean of clinical score: ~0.8
cKO: 7/9 incidence, mean of clinical score: ~1.7
This assay is finished, and day25 CNS infiltration total CD4+/total CD45+ cells, cKO exhibited ~1.5 fold more CD4 T cells infiltration than WT, statistically significant.
Female:
WT: 6/8 incidence, currently day12 post immunization, mean of clinical score: 0.8125
cKO: 1/8 incidence, currently day12 post immunization, mean of clinical score: 0.1250
This assay exhibited totally inverted phenotype of EAE severity between different genders, so the question is, was there any reported case of genetic models that could explain this phenomenon? The gene is not X/Y chromosome linked, and was floxed with CD4Cre. Or any other advice is welcomed.
Dear Guangyue Zhang
I briefly wanted to reach out concerning your inquiry. First I would like to ask, which statistical test you have used and would strongly advise a non-parametric for the assessment of non-normally distributed clinical scoring data. Secondly, it's not that surprising to find genes that control autoimmunity in a sex-specific manner, overall the phenomenon is well described for humans. With your experiment, specifically did you run a control group of ovariectomized females to check, if your phenotype is sex hormone dependent? If yes, you may have affected transcriptional control of your target gene by estrogen with your conditional KO construct. Please check our pre-print that pretty much describes your phenotype (in EAE and CIA) and should be published soon.
Preprint Polymorphic estrogen receptor binding site causes CD2-depend...
And here's the final paper including all revisions and additional experiments.
Article Polymorphic estrogen receptor binding site causes Cd2-depend...
All the best & good luck with your experiments,
Michael
Dear Michael Förster , thanks a lot for providing insight of this phenomenon. It's two way ANOVA, multi comparison, p=0.0003 for the column factor(between WT & cKO). We would consider about the excision experiment, and also, it's only performed once, we need to repeat. Thanks for the preprint, sincerely!
- Badges
- Science topic