Working with in vitro mycorrhizae, I first get them from pot culture. II extract them with sieving and gradient sucrose. At the end, I have a few ml of water with hundred of spores in it. I want to pick up the spore ONE by ONE, but I want to do hundred of in vitro trials with differents species in the next months. I want to look at the spores below microscope (60x) and pickup exactly those I choose for color, size etc. IT IS QUITE HARD TO PICK UP JUST ONE AND VERY TIME CONSUMING. Does someone have found or designed an efficient tool to pick up the spores one by one in liquid suspension ?
Hi
You can use painting brush with only one hair! I used it and it was useful!
Also you can use a pipette.
Good luck
Dear Louis Paré,
Don’t worry; it is very easy to pick up one mycorrizal spore at a time and it’s not time consuming--
· Use 10-µL Micropipette or less
· 40X bright field Stereo Microscope (widefield stereo magnification settings/ incident (top) and transmitted (bottom) lights )
· Dilute your 1ml sample with 3-4ml 1/4- Ringer's solution (not more than 20 spore/ml) and pour (3-4ml) it in 4.5 cm Petri dish
For picking spores, a pointed needle (even tooth-pick) may be used under stereomicroscope (40 to 60 X)
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