Bacteria in fecal samples grow even after being at -80 for long withjout cryopreservatives. Does that mean feces have natural cryopreservatives? Can we identify them?
The anti freezing molecules identification and isolation could open a new direction of research.
Faeces may or may not have natural cryoprotectants. Identification and isolation of natural cryoprotectans from faeces will definitely be an interesting field of research.
Besides, organisms especially from arctic and antarctic produce their own cryoprotectans (antifreeze compounds and antifreeze proteins) in their bodies to protect them from freezing damage during prolonged winter months.
Thus, it is advisable to study presence of cryoprotectants in bacterial cells thriving on faeces at -80oC.
Thanks, I was thinking of the same. Any suggestions on experiments that could be done to identify and isolate these cryoprotective molecules from the feces?
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