What are the general antibody concentrations in flow cytometry?

Hi everybody, I am trying to block Fc receptor by human IgG for a specific experiment (it's not flow cytometry), in an adherent cell, not a suspension. I know my primary antibody working...

20 September 2020 9,119 5 View

Can i report cronbach alpha for components with 2 items?

I developed a brief questionnaire with 20 items and 10 components, each component has 2 items. factor analysis confirmed the structure of the scale, and the total Cronbach alpha was 0.88. can I...

14 May 2020 5,893 3 View

Which statistical analysis is proper for randomized controlled trial research?

I have a randomized controlled trial research project. Eight training sessions were held for adolescents. I have a pre-test, post-test and follow up test after 30 days. I have two dependent...

05 March 2020 8,844 9 View

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

Has anyone ever experience for Aflatoxin determination in meat by HPLC?

29 December 2019 389 4 View

What is the correct output format of the htseq package?

Hi everyone I used the HTSEQ package to count the reads. The output of this package was as follow: Is this output format correct? Is the first column the gene id and the second column is the reads...

21 December 2019 9,326 4 View

Primer blast from NCBI?

Hello How we can do in Primer blast from NCBI that What size is made of PCR product with D-Loop Primers by using DNA samples from Cervus and Capra aegagrus?

04 October 2019 9,001 3 View

Why did my HUVECs growth rate reduced after transfection?

I did transfection using lipofectamin 2000 to get stable GFP positive cell line, but after transfection and puromycin selection my HUVEC growth rate reduced. I have not seen any colony displayed...

03 June 2019 5,109 5 View

Is system dynamics a proper tool for the simulation of Cash Based Interventions impact factors?

Can System dynamics simulate cash transfer narratives well, If we consider multiple factors such as education, health and economy?

30 January 2019 7,470 1 View

How can I remove the produced HCL during esterification reaction without adding base?

In the esterification reaction that I work on, HCl is produced.Adding base ruins the expected product since it reacts with the initial materials and forms a red byproduct. The bases that I tried...

23 January 2019 4,764 3 View

In cross frequency coupling, is it possible to taking one frequency from LFP and the other from EEG ?

28 June 2018 9,305 1 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

Apart from Nanosilica and CNT's what are the other nanomaterials readily available to use in concrete mixture?

Nanotechnology in concrete materials

01 March 2021 3,379 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

How to calculate refractive index (n and kvalue) against energy (in eV) data for alloyed nanomaterials through Independent Phase Approximation method?

Suggest me the method or some other software to calculate the above said data for nanoparticles.

21 February 2021 3,350 7 View

What is the best substrate for exfoliating few layers of hexagonal boron nitride (hBN) using traditional scotch tape method?

Unlike graphene, it is usually very difficult to exfoliate few layers (~10 nm thick) of hBN on SiO2 substrate due to lip-lip interaction at the edges. Au substrates have been proven very useful...

16 February 2021 2,635 2 View

Having 2 different substrates under the same deposit conditions (TiN coating) why it is possible to obtain different colours in the 2 dif substrates?

Coating is made by same deposit conditions. substrates are HSS Drill bit and Shim SS 300 series. On the drill bit we are getting some golden color, however on the shim we got no color it is like...

08 February 2021 9,383 7 View

Is it possible to find out the wettability and contact angle of water on the rough or corrugated substrate?

First of all, whether it is possible to identify the presence of water and its amount on the Silicon substrate by using only FT-IR spectroscopy. Can ATR-FTIR spectroscopy find out the wettability...

03 February 2021 9,085 4 View

Will you suggest recent literature regarding photocatalytic applications of nanomaterials?

Dear esteemed researcher, Please, suggest recent literature links related to photocatalytic applications of nanomaterials. Regards, Dr. Vinaya Tari

26 January 2021 3,930 9 View