Hi Nigus

in this paper you'll find sequences of primers to amplify and sequence the Nad5 gene from your sample.

Article Distinguishing Echinococcus granulosus sensu stricto genotyp...

if not sufficient, use the NCBI database with right species to get the sequences and design your primers:

https://www.ncbi.nlm.nih.gov/search/all/?term=echinococcus

all the best

fred

I think enough literature available.

Check the follwoing links and attached.

Good Luck!!!

Validation of a one-step PCR assay for the molecular identification

of Echinococcus granulosus sensu stricto G1–G3 genotype

(https://link.springer.com/content/pdf/10.1007/s11033-019-04625-w.pdf)

(https://www.ncbi.nlm.nih.gov/pmc/?term=echinococcosis+mitochondrial+genome)