As NaCl substitute in Na+ free buffers, NMDG (N-methyl-D-glucamine) is firstly prepared as a chloride solution or can we directly titrate with HCl in the final buffer?
Hello Rona
In principle it makes no difference whether You start with the pure
N-methyl-D-glucamine and neutralize it with HCl, or make the buffer from a mixture of pure base and its corresponding chloride.
Maybe solubility of the pure base could be an issue, but besides that it is merely a matter of convenience.
Thank you for your answer! You are right, solubility of the pure base was an issue but titrating with HCl solved it. One more question, do you happen to know the stability of this compound? I used the buffer containing NMDG in my cellular experiments, and over a period of 4 days (stored in refrigerator), under the same conditions, it gave quite different results. On the other hand, experiments done with the same buffer components and replacing NMDG with NaCl, gave quite consistent results. Thank you in advance for your time and cooperation!
Hello Rona
I am sorry, but I have no specific knowlede regarding the stability of NMDG.
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