N- and C-terminal residues?

Yasser Almeida Hernandez @Yasser_Almeida_Hernandez

01 January 2014 1 6K Report

Which amino acids are the most frequent in the N- and C-terminal positions? Is there some work about this issue?

I don't know of a specific study of that topic, but it is fairly easy to do yourself. Just download the uniprot database (just the fasta sequences) and extract the first and last characters of each sequence. Easy to script.

Badges
Science topic

More Yasser Almeida Hernandez's questions See All

How can you improve primer design for archaea gene cloning?

I am designing primers to clone membrane protein homologs from several archaea organisms in E. coli. The templates sequences are very CG enriched, resulting in high Tm temperatures, %CG and...

11 December 2014 7,307 2 View

What Kd cut-off values are the accepted for consider a protein-ligand complex with a low, medium or high affinity?

Kd cut-off values

11 December 2013 5,744 6 View

Which value of timestep would be better for a 40 ns MD simulation with NAMD?

I am working with a 153 aa protein.

11 December 2013 9,161 6 View

NAMD vs GROMACS?

I am working with a 153 aa protein to evaluate the influence of point mutations in the stability and folding, by molecular dynamics simulations. To this task, which package would you recommend,...

11 December 2013 3,884 0 View

Tools to analyze protein-protein interactions (PPI)?

I have a list a several proteins and I want to search for interactions between them directly by a second or third protein. Which are the more suitable tools to do this? I've used Cytoscape for...

08 September 2013 1,622 5 View

How to get ontologies from a PDB IDs list?

I have a list of PDB IDs list and I need to get ontology data to verify any enrichment of protein family, function, cellular localization, etc. Which tool is the more suitable?

11 December 2012 9,127 1 View

Lysosomal vs. Nuclear EGFR Trafficking

Upon Ligand binding (EGF, TGF-alpha) EGFR is internalized and finally sorted to degradation in lysosomes, or translocated to the nucleus, where it regulates the expression of several genes. Which...

05 June 2012 9,590 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View