hello all,
A research group and I are finding out the antimicrobial activity of A. fumigatus over susceptible and resistant strains of M. tuberculosis.
We are proceeding the MOPS method using the Aspergillus' extract in DMSO 5%. However it is not working well, so we read about a PRE-TREATMENT (an still unknown reagent for us) to be applied with the liquid medium in MOPS test.
So, this REAGENT can disolve the lipidic bilayer of Mycobacterium and the extract can diffuse and perform much better.
Besides, this reagent mustnt have antimicrobial activity by itself; in order to avoid a bias.
I look forward for your help please!
Thanks in advance
"Pre-treatment" is not a single reagent, rather just the denomination of any chemical used before the definitive reaction, either to activate the chemicals being studied, remove impurities, activate dormant molecules or zymogens, digest or dissolve complex molecules into simpler, more reactive ones. Substances like ammonia, sulphuric acid and even water are used as pre-treatment reagents. Commercial kits often come with various chemicals which may be referred to the the pretreatment reagent in the manual.
Some diagnostic kits for TB depend on the extraction of nuclear material and employ such reagents. It might be a useful avenue to look into. Good luck!
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