I treated them with 70% alc, 2.5% NaOCl, 0.1 HgCl2, and 1g/l bevastine. These chem were used in diff combinations and for diff time periods. Infection still occurs. Please suggest any other way of disinfection and sterilization.
Ankita Dave
Was the forceps you used to move the sterilized seeds to medium sterilized too? We autoclave our forceps beforehand. Also, we dip it into 70% ethanol, flame it briefly before touching sterilized seeds (after cooling down from the flame). Also, was your medium sterilized properly? The autoclave worked 100% properly?
Wipe the stems of tobacco with 96% ethyl alcohol and then additionally sterilize with a 0.11% aqueous solution of sublimate. Pieces of the stem are immersed in a solution of sublimate for 15 minutes. Tobacco stalks are washed with sterile water from sublimate. In the last glass, the stems are left and taken periodically for work.
Yuan-Yeu Yau The forceps were sterilized properly and the medium was properly autoclaved. The issue is that the seeds are infected with fungus and usual surface sterilization is not working.
Fresh, healthy seeds generally do not get infection and even do not require any surface sterilization. However, in certain cases especially stored or old seeds, due to loss of viability/dead tissue and/or storage condition, the seeds get infected within the seed coat and can not be sterilized by usual surface sterilization.
Check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6679225/
https://www.frontiersin.org/articles/10.3389/fpls.2012.00268/full
Also check https://www.researchgate.net/publication/354248884_Fungal_Growth_and_Mycotoxins_Production_Types_Toxicities_Control_Strategies_and_Detoxification
https://www.researchgate.net/publication/351828255_Mycotoxins_Affecting_Animals_Foods_Humans_and_Plants_Types_Occurrence_Toxicities_Action_Mechanisms_Prevention_and_Detoxification_Strategies_-_A_Revisit?_sg%5B0%5D=dF7kRBtfbR6aTLouU3UdPhx4Bqbk5gSzD0apWozRG3BcECDxCH3XMH7Kkf2fpvNMae1ZsHaLOMH_6PtKwwsi6NJwbSoXFNIgRlYQ4F1g.9Js0i4cgVXtypCzNgudTnNoT0ZD9hE7XyKp2snp48ImaSBXA-kX2FNmCz6enHuZw4QNB63ogygc7PSTMBIm5Rw
https://www.mdpi.com/2077-0472/8/7/88/htm
Wipe the stems of tobacco with 96% ethyl alcohol and then additionally sterilize with a 0.11% aqueous solution of sublimate. Pieces of the stem are immersed in a solution of sublimate for 15 minutes.
https://www.researchgate.net › post
My transgenic tobacco seeds have been infected with fungus. what ...
https://wew.nature.com.articles
Enhanced protection against fungal attack.
https://pubmed.ncbi.nimnih.gov
Transgenic tobacco plants overexpressing chitinases of fungal.
Check on this body.
https://portal.ct.gov/-/media/CAES/DOCUMENTS/Publications/Circulars/C181pdf.pdf?la=en
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