It is not possible to obtain a nanoparticle size of 0.34 nm through X-ray diffraction (XRD) analysis. XRD is a technique used to determine the crystal structure and lattice parameters of materials by analyzing the diffraction pattern of X-rays that have been scattered by the atoms in the sample. The size of the nanoparticles cannot be directly determined by XRD.

In addition, the result of 0.34 nm that you obtained from the scatter equation is not consistent with the typical size range of silk fibroin nanoparticles, which is typically between 10-200 nm. It is possible that there may be an error in the calculation or interpretation of the data.

To determine the size of your silk fibroin nanoparticles, you may consider using other techniques such as dynamic light scattering (DLS) or transmission electron microscopy (TEM). These techniques are commonly used to determine the size and size distribution of nanoparticles.

Sadiqul Hasan

Silk fibroin, like most polymers, is amorphous. Therefore, the diffraction pattern has wide peaks (halos). To determine the size according to the Scherer formula, crystalline samples are used. Perhaps you neglected it. That's why you got that size. If the peak is narrow, then you have determined the approximate size of the crystalline part of silk fibroin.