Plant media get cracked after inoculation even if I adjust PH 5.8-6.5. what can I do My media composition is Ms media(with sucrose)+Growth regulator+Agar....Anybody plz guides me on what can I do ???
Dear Suraj Pandurang Devkar what do you mean with cracked? First, it's important to know agar brand and concentration; also, the autoclaving procedure. Why was the adjusted to 6,5?
Respected Ricardo Julian Licea-Moreno sir, cracked means when I inoculate explant, within 1-2weak media get cracking see the image attached I used HI MEDIA Agar brand the desired PH I adjusted is between 5.8 to 6.0 according to past protocols.
Then, seeing th picture two probable causes might been acting: high agar concentration, and/or high rates of evaporation (it usually happens when untight vessels are used). A third factor could it be also posible, the use of desiccated culture medium, but it could happens when old batches are used.