my bacteriophage stock was sent for whole genome sequencing and the report said it couldn’t be sequenced properly because of DIN= 6.7 and conc of DNA is 1.2 ng/ul …
can you help me how to increase the concentration of Phage stock properly and in short time and how to increase DIN value?
what does this diagram tell about my phage?
and finally what is the minimum conc of DNA should be targeted before NGS whole genome sequencing??
first you should prepare high titer phage and than you can use norgen phage DNA extraction kit. And in final step add 50ul final buffer to get DNA and when you get DNA again put that liquid on the column it will increase the conc
Thank you for your answer
can u recommend for me a protocol for increasing phage titer in a short time ?
Make a complete lysis plate and add 4ml sm buffer on the plate. place at 4 degree overnight than pick the sm buffer by syringe and filter it with 0.22um
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