The Akaike information criterion of the random forest model ?

Please, how to calculate the number of model parameters of the random forest model to determine the Akaike Information Criterion (AIC) using this following expression: AIC = n * log(MSE) + 2 *...

27 January 2021 816 6 View

Why the life span of erythrocytes of mouse is shorter than other mammalian erythrocytes?

Hi, everyone, I have one question. Why the life span of erythrocytes of mouse is shorter than other mammalian erythrocytes, including that of humans and other veterinary species? Any answers are...

26 March 2020 6,564 5 View

What tests (not quenstionnaires) can be used to measure social interactions with peers in adolescents?

Hi, I am a student whose project is about social behavior. I want to know if there is any test can be used to meausre social interactions with peers in adolescents. The test is good to be a game...

06 February 2019 6,686 4 View

How can I make ceramic particles be negative charged in the aqueous solution?

I am studying anodic oxidation of Ti and devoting to coat hydroxyapatite (Ca10(PO4)6OH2) on Ti. During anodic oxidation, Ti was set as anode and a Ti oxide formed on the Ti surface. I would like...

06 September 2015 8,466 3 View

Question about the expression of one heterogeneous protein in the chromosome of bacteria.

Hi, everyone, I want to insert one heterogeneous protein gene into the chromosome of one bacterial strain, How can I choose the locus in the chromosome for insert heterogeneous gene to let that...

01 January 1970 481 3 View

From your perspective, the reduction of synthesis rubber can affect the product quality and its chemical properties or not?

I think the chemical properties of synthesis and natural rubber are partially different in term chemical bound.

01 January 1970 1,415 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

QTL, recombination frection?

22 February 2021 2,588 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View