Anyone culturing CTLL-2's? Cell density tips?

01 February 2016 8,157 2 View

How to quantify protein concentration......?

I have carried out some nanoparticle treatments on a set of cells, which I plan to use for western blot. Of 7 treatments, one set of cells has turned red, while another dark purple. I think this...

31 December 2015 5,430 8 View

What binds to gold?

11 December 2015 5,071 7 View

Any advice on Dendritic Cell - T Cell CoCulture methods?

Hi, I'm currently setting up T-DC co-cultures and am looking for advice. T cell line is IL2 dependent. T cell media contains IL2 (amongst other supplements), although DCs produce IL2. Any advice...

07 August 2015 6,616 11 View

Has anyone had issues with western blot samples seeming to exclude ECL reagent?

I am interested in confirming whether a targeting peptide we have developed is successful in binding its target receptor protein. To do this, I have been running pull down assays to detect...

06 July 2015 7,554 9 View

How can I confirm streptavidin - peptide binding and concentration?

05 June 2015 1,064 6 View

Is anyone using Abcam Anti-CD40 ab13545 for Western blotting?

I have just begun western blots looking at CD40 in immune cell pull downs. The antibody daasheet states the band will be apparent at 41kDa, although the predicted size is 34kDa. I am not too...

05 June 2015 5,399 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

No title

02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View