8 Questions 18 Answers 0 Followers
Questions related from Brittany Wingham
If anyone would be willing to recommend or share a cell line I would be incredibly grateful!!! Thank you :) Brittany
04 April 2016 5,201 1 View
Hi again! I've just started growing CTLL-2 IL2 dependent T cells in culture, and need quite high numbers. The ATCC recommendations for culture are to subculture actively growing cells BEFORE they...
02 February 2016 8,253 2 View
I have carried out some nanoparticle treatments on a set of cells, which I plan to use for western blot. Of 7 treatments, one set of cells has turned red, while another dark purple. I think this...
01 January 2016 5,526 8 View
I'm looking at gold nanoparticle binding on the cell surface, as well as cell uptake. I planned to use TEM, but ours is currently out of service and I am keen to confirm my results. Is there any...
12 December 2015 5,173 7 View
Hi, I'm currently setting up T-DC co-cultures and am looking for advice. T cell line is IL2 dependent. T cell media contains IL2 (amongst other supplements), although DCs produce IL2. Any advice...
08 August 2015 6,811 11 View
I am interested in confirming whether a targeting peptide we have developed is successful in binding its target receptor protein. To do this, I have been running pull down assays to detect...
07 July 2015 7,627 9 View
I have just begun western blots looking at CD40 in immune cell pull downs. The antibody daasheet states the band will be apparent at 41kDa, although the predicted size is 34kDa. I am not too...
06 June 2015 5,472 2 View
Hi, I have been looking at formation of a peptide multimer bound to a streptavidin scaffold. The peptide is biotinylated, so should bind inherently with the streptavidin backbone. Currently, I...
06 June 2015 1,168 6 View
