No smears on gel after cDNA synthesis ?

Hi all, I have a problem with detection of cDNA synthesis where when I put it on the gel , there were any smears so what the meaning of that and what can I do? Thanks

21 December 2020 1,126 2 View

Is e.coli ok at Electroporation at 30C?

For reference: I am transforming a newly assembled plasmid what happened: I accidentally incubated my electroporated e.coli cells on the shaker at 30C for 1 hr rather than 37C and did not...

02 December 2020 7,133 5 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

How to develop a framework/model and present it in an analytical form?

Dear All, I am conducting a study on DBD actuators and I'm trying to develop a framework for actuators using plasma-based metamaterials. In order to reach this objective, I need to learn how to...

01 June 2020 3,864 2 View

Duplex PCR Target A & IC & target B & Ic works great. When multiplex the three together A FAM , B HEX, IC Cy5 , A & IC fine, B curve plateau strange?

The curve by B in Hex starts great but the plateau looks like zigzag. I tested different primer concentrations and different annealing temperatures. We use Lightcycler 480 II. Color compensation...

10 May 2020 7,879 3 View

Are ACEIs underdeveloped in children?

COVID19 shows less affection of lung in children . this may be due to underdeveloped or decreased expression of ACEI in lungs of children taking in consideration that as age increase from 5...

28 March 2020 2,448 2 View

Solution not converged at time ........... in ansys?

I am doing four point flexural text on simply supported beam using ANSYS APDL first with steel plate for loading and supporting and then without it. In both cases there is a problem of non...

19 March 2020 4,775 6 View

Can we find the Link between Teaching Methods and Achievement in Math in Computer-Assisted Elementary Schools?

I published a paper in: Creative Education, Published Online http://www.scirp.org/journal/PaperInformation.aspx?PaperID=47205 The Link between Teaching Methods and Achievement in Math in...

14 February 2020 1,408 4 View

How to detrend a series in Stata when it has a stochastic trend?

08 February 2020 1,803 3 View

What is the editorial office email of this journal?

I submitted an article and received an email stating that I need to fix the word count to 5000 or less., though the word count in my manuscript is 4330. I sent back an email in this regard and...

06 October 2019 9,154 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View