MTT assay in 3D gel?

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Ranjith Kumar.R

Dr. Narayanan,

You can consider disrupting the matrigel in order to dissolve the formazon crystals with acidified isopropanol instead of DMSO.

Narayanan Janakiraman

Thanks Ranjith for suggestion

Steingrimur Stefansson

Hi Narayanan, you could use trypsin and/or proteinase K to hydrolyze the Matrigel, spin out the cells and then do the MTT assay.

Thanks Steingrimur for the good suggestion