I am measuring a gene which is involved in differentiation of a cell. During differentiation its level is high, compared to undifferentiated state. Upon terminal differentiation of cell, mRNA level goes down significantly compared to differentiating cells.
I want to know, whether if I measure the same at protein level, will it be the same difference? Does all mRNA gets copied to protein? At the moment, there is commercially available antibody to probe at protein level.
No doubt very interesting question,but here i want to discuss one thing that mRNA is actually copied from DNA ,but mRNA copying actually depends upon the cells/tissue/organ's product need and then various factors get orchestrated to decode the mRNA into corresponding protein so mRNA varied during various stages as you have mentioned that it decreased during terminal differentiation.
And your next question's answer have many solutions that mRNA level may be silenced by mi RNA or Si RNA so its value may be declined.Another chance may be that corresponding gene may be alternatively spliced into other type of transcript and thus other type of protein ----as one gene many proteins or polypeptides proved after genome sequencing.
And in last questions yes Agilent technologies have such type of instruments-in proteomics .
OK,,,,,,all the best dear
Chandan, also see the prior post on this at:
https://www.researchgate.net/post/mRNA_versus_protein_expression_Why_inverse_correlation?ev=tp_feed_post_xview
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