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Questions related from Chandan Kadur Nagaraju
I am isolating fibroblasts from heart tissue. I plate them onto T-75 flask. Usually, yield was always consistent. Since one month, cells start to die either at the end of P0 or after...
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I am isolating cells from heart tissue. I would like to stain them and FACS them for different markers. However, I have many samples coming together and simply don't have enough time to stain them...
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I have isolated total RNA from mammalian cell culture samples. I used a Qiagen Kit. I have checked the conc and quality of RNA by Nanodrop. Ratio of 260/280 was between 2.01 and 2.09, and 260/230...
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I want to quantify the Angiotensin-converting enzyme (ACE) in cell cultured samples. I prefer to do ELISA to quantify ACE. Which lysis buffer should be used to lyse the cells? The supernatant...
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I am using Applied Biosystems 7500 Fast system for qPCR. I am using 10ul sample per well. Today I run the sample, however, I forgot to change the sample volume to 10ul from 20ul in the software...
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