mRNA expression of an enzyme produced by bacteria in human feces?

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ANOVA/Mixed models in R : how to find the correct formula, interpret the results and what post-hoc comparisons?

Hello, I am looking for an advice regarding my experimental design and what statistics I should do. My experimental design: I have 3 cell lines (A, B, C) and from each I do 3-4 differentiations...

29 January 2024 3,498 13 View

What is the role of Digital Touch Points in the 5 stars Hospitality Customer’s Journey Industry ?

The hospitality industry has undergone a significant transformation in recent years, with an increasing emphasis on providing a seamless and memorable customer experience. In the pursuit of...

27 September 2023 4,042 1 View

What is the reasoning behind the use of TRFs?

Hello, I'm about to join a team working on auditory speech perception using iEEG. It is planned that I will use Temporal Response Function (TRF) to determine correlations between stimulus...

13 September 2023 2,478 3 View

One step in achieving a good quality control in the lab is risk assessment. What are the internal quality control measures done to limit these risks?

Quality Control: Risk Assessment

03 September 2023 8,024 3 View

Are there any risk in involving and applying the use of artificial intelligence in the clinical laboratory especially when obtaining the result?

As the world is advancing, new and advance technologies are being invented. Artificial intelligence (AI) is the ability of computers to carry out operations that ordinarily requires human...

02 September 2023 1,905 3 View

Can I dissolve collagen IV powder in PBS for flask coating?

Hi, I purchased collagen IV powder from Sigma and the website says to dissolve in acetic acid, PBS or water. As I didn't have acetic acid I tried to dissolve it to 1mg/mL in PBS but the collagen...

05 July 2023 3,420 1 View

How to do a TUNEL / cell death assay on whole-mount E11.5 mouse embryo?

I am using Roche In Situ Cell death detection kit, AP. I know this kit is designed for sections but it seems to be possible to use on whole mount mouse embryo though no literature I could find...

14 June 2023 9,992 0 View

Can i conduct a comportmental study with before/after design without control group ?

Hi! I would like to evaluate the causal effect of a treatment, that i will indirectly measure through psychological questionnaires. Also, i have no control group (this is a natural experiment). I...

23 April 2023 2,672 2 View

Anyone knows the issue with Micriphone circular array beamforming I described below?

Hello, recently I am working on the design of microphone array beamforming using convex optimization.I designed the circular array beamforming with the sidelobe suppresion of 20db, 30db, 50db and...

06 March 2023 1,872 2 View

How many PCA dimensions should I keep ?

I am exploring the coordination of plant traits through PCA. The first dimension explains a high proportion of inertia, so I used a broken stick model to help with the decision of how many...

26 February 2023 553 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View