Hi all
Just received a fresh batch of Illumina MiSeq data and its giving me troubles in the very first step! I am using MOTHUR v 1.37.1 and a large number of files are showing error/segmentation faults while using the command; make. contigs-
Eg:
mothur > make.contigs(ffastq=processed_ML-26_S24_L001_R1_001.fastq, rfastq=processed_ML-26_S24_L001_R2_001.fastq)
Using 1 processors.
M00933_150_000000000-AU71L_1_1101_10003_11841/1 is in your forward fastq file and not in your reverse file, please remov e it using the remove.seqs command before proceeding.
Making contigs...
Segmentation fault
Is it because the reverse read is not long enough and the program is unable to make a contig or am I missing something extremely trivial? Also thoughts on using trim.seqs before make.contigs? Will that salvage the data?
Any comments/suggestions will be welcomed.
Many thanks