06 August 2016 4 10K Report

Hi all

Just received a fresh batch of Illumina MiSeq data and its giving me troubles in the very first step! I am using MOTHUR v 1.37.1 and a large number of files are showing error/segmentation faults while using the command; make. contigs-

Eg:

mothur > make.contigs(ffastq=processed_ML-26_S24_L001_R1_001.fastq, rfastq=processed_ML-26_S24_L001_R2_001.fastq)

Using 1 processors.

M00933_150_000000000-AU71L_1_1101_10003_11841/1 is in your forward fastq file and not in your reverse file, please remov e it using the remove.seqs command before proceeding.

Making contigs...

Segmentation fault

Is it because the reverse read is not long enough and the program is unable to make a contig or am I missing something extremely trivial? Also thoughts on using trim.seqs before make.contigs? Will that salvage the data?

Any comments/suggestions will be welcomed. 

Many thanks

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