PAGE was done using Sigma-Aldrich TurboMix Bis-Tris Gel Casting Kit (TMKIT-10, TMKIT-60),
we have cast total of 2 gels into this experiment, while in the end of PAGE, bands of protein ladder can be clearly seen, which from the highest 180 kDa to 17 Kda has been separated.
The transfer has been performed later, we used constant voltage 30V for 1 hour and left in a 4 degree environment.
The result showed as attachment, the diffused red band was used to estimate around 72 kDa protein, which can't be distinguish with other bands, and this is the result in one of two PAGE gels, while other one showed normal band pattern.
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