Why does DMSO become liquid with extended storage at -20C?

I noticed that drugs or reagents dissolved in DMSO and stored at -20C become liquid after a year or two of storage. Does anyone know why this is and if it affects the effectiveness of the...

30 September 2020 7,901 2 View

How do I deal with small sample size and nonnormality in SEMs (AMOS or R)?

Hello! I have a sample size of N = 70 at the moment, hoping to aproach 100. My Model has 21 parameters to be estimated. The Variables are all not normally distributed. Now the options that I know...

20 July 2020 9,849 6 View

Does anyone recommend a PMD simulation code for DP-QAM?

Does anyone recommend a PMD (polarization mode dispersion) simulation code (MatLab, Python, or C) for single-carrier dual-polarization quadrature amplitude modulation (DP-QAM)?

05 July 2020 6,274 1 View

What is the Full Spectrum of Neuroscience Research Techniques that Exist?

Hi! I graduated with a Neuroscience and Psychology BSc in 2016, and my final year project was a bench-lab in vitro study of omega-3 oils applied to fluorescently-stained cortical neurons. I...

26 May 2020 4,650 6 View

Which cell culture inserts do you use?

In our experiment we use TC treated 96-well plates from Eppendorf and coat our protein of interest. After coating we add monocytes and get our readout. Now, we want to add activated T cells to the...

02 March 2020 6,065 2 View

What is the most efficient way to exchange buffer in protein soltuions?

Hy everyone, I'm facing some troubles when changing the buffer of my protein solution. I'm doing a 2-step purification of my protein, first on a nickel-column and second on a heparin column....

31 October 2019 793 4 View

Why does dynamic surface tension of gelatine solution increase before it decreases?

02 December 2018 949 3 View

Why are there a huge number of dead cells in my culture ?

Hello, I am culturing 293 cells for onward transfection. Unfortunately, I have observed that the cells are not multiplying as fast as they used to. Besides, i see several dead cells in the T25...

26 June 2018 4,274 5 View

Why does my SDS PAGE gel bubble and form a bell shape towards the bottom of the tank?

Hello, I am doing some SDS PAGE. Now, i am baffled by the fact that my gel bubbles at the bottom and there is a bell shape in the sample under separation, towards the bottom of the gel ( as...

15 May 2018 8,675 2 View

What is the best method to assess viability for adherent breast cancer cell lines?

For my project, I need to set up a viability method to see if inhibitors are responding. We currently don't know which method suits best and because there are so many we don't know which one we...

04 April 2018 2,737 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View