I want to take a sample every day from shake flask to measure the dry weight of biomass, but the phanerochaete chrysosporium is not homogenized.
In order to measure the fungal biomass/dry weight, you can grow the fungi for 3-4 days on shaking condition and then filter the media through Whatman filter paper No. 1. After this keep the filter paper along with fungal filterate and dry it at 40-50°C for 42 h-72 h in an air incubator. After it measure the dry weight of this and also the weight of another filter paper applied in the same procedure but without any mycelia (control). Minus the dry weight of later from former and it will give you the dry weight of your mycelim. (Singh et al., 2012, PLoS ONE 7(3): e33128)
Kunai´s answer is correct, but you should dry the filter paper alone and use the same paper to recover your biomass... thereafter you make the measures of weight... although be of the same box, filters papers change in weight and dry fungal biomass weight can be very small and you will no be able to measure the real weight with this method
After seperating the supernatant with filter paper, or cheese-cloth, I use to freeze dry the biomass. That worked well.
firstly dry the whatman filter paper to remove misture content because moisture itself gives a overweight.after that filter your sample and put whatman filter paper for complete drying.measure the weight of filter paper.
Estimated weight = total weight - weight of filter paper taken as a control(without moisture)
good luck.
Hi! All. while going through your informative conversation, a question poped up in my mind. Could anyone tell which grade filter paper could be used, qualitative or quantitative. Any specifications. pl. tell if any?
Does anyone have a simple and low cost method for measuring fungal biomass in the presence of undigested lignocellulose? I want to measure the amount of fungus produced, but cannot use high-tech methods that require sophisticated equipment or expensive chemicals. Thanks
Answer to Dr. Robert F. H. Dekker: We measure fungal biomass using method described in Bioresource Technology 241 (2017) 652–660.
Answer to Fatemeh Khodaparastan: the comments above are correct. However if you intend to measure biomass dry weigh every day it will be better to use whole flask instead a sample
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