Expression of periplasmic proteins

I want to clone a protein from a gram-negative bacterium that has a natural periplasmic signal peptide. Is it ok to delete the signal sequence, put the N-terminal amino acid directly next to a...

14 June 2013 9,849 1 View

How do I design primers to express a protein with C-terminal His tag?

I'm trying to clone and express a protein in E.coli using pET26b or 33b vector. I am designing primers using the NcoI and XhoI restriction enzymes. I want to know how to design primers to include...

26 May 2013 9,746 9 View

How to measure beam radius over some distance in Lumerical fdtd (Collimation evaluation)?

31 January 2021 6,354 2 View

How can Plasmonic nanoparticles with different shapes be simulated using FDTD if they are imbedded in Organic solar cells ?

I need help on how can i simulate Organic solar cells with Plasmonic nanoparticles of different shapes using Lumerical FDTD . Is there any body familiar with such simulation and can share...

26 January 2021 4,188 3 View

Why transmission power drops with Lumerical FDTD simulation time?

Hello everyone, I am trying to design a ring resonator in FDTD. I simulated the design and found transmission power dropped by increasing simulation time. Once I increased the simulation time the...

21 January 2021 4,872 4 View

Is it possible to generate interference pattern ( interference fringes) using simulation program? what is the name of this program ?

I want to do an experiment using two optical fibers then flowed by (Y) coupler, at the single end of the coupler there is a CCD camera to see the fringes ... what is the program that will give...

23 December 2020 6,963 2 View

How can I fix the `engine error` with no error message in Lumerical DEVICE software when it only shows directories instead of error message?

When I run my simulation in Lumerical DEVICE, it shows an error entitled `engine error` after reaching the simulation to 100 percent. When I right-click and chose 'job details`, it only shows the...

22 October 2020 4,973 1 View

How to perform Multipolar Analysis in a resonant metamaterial structure?

As multipolar analysis is a key to find out contribution of different dipole moments, quadrupole moments causing the resonance in a metamaterial. I want to know the method, software and...

12 October 2020 7,012 6 View

How to transform this implicit FDTD iteration equation into the matrix equation?

11 October 2020 9,042 1 View

Coupled partial differential equation using crank Nicholson implicit method ?

The problem is let's say if I finite-difference one out of the many equations then through clank Nicholson, we separate the (t+1) time step left-hand side and (t) right side, then there are some...

02 August 2020 9,837 5 View

What is the problem of zero output in the FOR loop in MATLAB?

Hello Dears This problem is related to the MATLAB link with LUMERICAL. I have used the MATLAB link feature with Lumerical software, Also in MATLAB, I have written a script for sweep Jsc according...

16 July 2020 6,500 3 View

How can I calculate group index of one dimensional photonic crystal by using Rsof?

12 July 2020 6,040 2 View