How to select the number of nearest neighbors in relief algorithm for feature selection?

For feature selection of a binary class dataset, relief algorithm chooses an observation randomly and then selects nearest neighbors of that observation from both classes. Selecting number of...

09 February 2021 4,991 2 View

What is the use of vaccination without follow-up tests for assessing the corresponding antibodies ?

Shouldn't every individual who takes COVID-19 vaccination undergo periodic review to the study the state of corresponding antibodies? Individuals who do not produce any symptoms of COVID-19 after...

23 January 2021 6,170 2 View

"WaitFlag Error" in SR785 Dynamic Signal Analyzer followed by automatic reboot?

My SR785 Dynamic Signal Analyzer keeps rebooting after showing a "WaitFlag Error" in between measurements. I have been unable to find any mention of this error in the service/user manuals...

23 December 2020 8,427 2 View

Is there a solvent for polypropylene at room temperature?

I want to dissolve polypropylene for making a solution so as to make fibres at room temperature. I have tried toluene and xylene but without any success in dissolving.

06 December 2020 8,479 2 View

No title

I have tried using PowerSoil Pro kit from Qiagen with slight modifications- incubating at 65 degree for 10 mins after adding solution CD1, increasing the number of washes with CD5 to 3 times and...

27 October 2020 7,782 3 View

Why Ripple filter tuned at half of the switching frequency?

Dear Sir/Madam, Why ripple filter tuned at half of the switching frequency? what are the reasons? Kindly answer this question.

23 September 2020 6,204 1 View

How can I add alloying element to a material in Ansys Maxwell?

Hello, I am trying to perform an electromagnetic simulation in Ansys Maxwell. In my case, I have a rectangular plate made up of Aluminium and I want to add small MnB (Manganese Boron) particles...

01 May 2020 4,637 1 View

Can someone please help identify this species?

Hi, Can someone please ID this species? Location: Manipur, Northeast India.

26 April 2020 8,226 4 View

Can two analyses from the same study (two different time points) be used within the same meta-analysis outcome?

I have a study which reports PTSD diagnostic status at both 3 and 6 months. In my meta-analysis of the intervention, both 3 and 6-month assessments fall within the same outcome assessment (for...

26 March 2020 2,432 3 View

Indicator variable - Pooled regression results vs data partition results?

This is the Base regression: Y = b0*X1+b1*X2+error term I am using an indicator variable Z which takes value 1 for years 4 to 6 and value 0 for years 1 to 3. I am trying to analyse the impact of...

05 March 2020 9,865 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

What is the reason, we got both test and control band on LFA in negative sample?

I am working on Lateral flow assay for virus detection by carbon conjugated polyclonal antibody. We got a high intense band in both test and control line on LFA in negative sample used as buffer....

11 February 2021 6,558 2 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View

During an anti-His capture ELISA has anyone had issues with histidine residues from secondary antibodies non-specifically binding?

Hi, Hope you can help. Currently been running some sandwich ELISA assays where I coat with anti-His, block and then capture His-tag protein and then sera and then anti-bovine monoclonal...

07 February 2021 7,101 1 View

How do I keep my prefixed (PFA) frozen tissue sections from lifting off the slide?

I'm trying to stain mouse lung tissue IHC-IF I fix in 4% PFA overnight immediately upon harvesting tissues Then dehydrate/cryoprotect in sucrose gradients 15% to 30% Then freeze in OCT media...

06 February 2021 2,336 3 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View