I am measuring ROS production with DCFDA and using rotenone as my positive control. However, I can not find a concentration that will inhibit ROS production and the literature gives a very wide range.
I recently used MitoTEMPO at a concentration of 500 µM in J774A.1 to interrupt inflammasome signaling, as described in the paper linked below:
Article K+ regulates Ca2+ to drive inflammasome signaling: Dynamic v...
In context of this topic, I would like to bring to your attention a recent review of our group, "Functions of ROS in macrophages and antimicrobial immunity".
In this review, we give an introduction to ROS and their sources in macrophages, summarize the versatile roles of ROS in direct and indirect antimicrobial immune defense and provide an overview of commonly used ROS probes, ROS source inhibitors and ROS scavengers (also the difference between ROS scavengers and antioxidants, which are not synonymous, is explained).
If you like, please have a look at:
Functions of ROS in Macrophages and Antimicrobial Immunity
February 2021, Antioxidants 10(2):313
DOI: https://doi.org/10.3390/antiox10020313
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