Hi, I am testing if some compounds affect several mitochondrial complexes. For mitochondrial Complex II we have used an abcam kit. Here I attach an image of the graph I have obtained. Even though I see the compounds are doing something to the complex, when the Slope is calculated (as it is described in the kit protocol), there is almost no difference between the control and treated samples. How I should identify this "translation" of the curves to the right, without effect on the slope?
Thank you very much for your help!
the process can be described with differential equation.
the process for CT has an inflection point at 280 sec. CT process is established near 650 sec.
you can compare the processes with distance functions like Mahalanobis distance function and FDR - Fisher discriminant ration.
the processes 1 and 2 seem not finished, but they also can be described by differential equation.
THE PROCESSES ARE NOT LINEAR
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