I intend to analyse the expression pattern of 25 selected miRNAs on 250 PCI-CAD patient to identify a miRNA and/or specific miRNA signature for the identification of AMI. I have calculated the ΔΔCt using the appropriate formula and have the up or down regulated miRNAs.
Now for subsequent analysis I need to know the appropriate bioinformatic tool in order to determine
1. the potential mRNA targets for these up or down regulated
2. strongly dysregulated pathways
3. combined effect of up or down regulated miRNAs in cellular function
Also the best way to represent the data in either table or Figure format
Hi,
Using Target Scan web portal ,you can get the potential targets for your identified miRNAs . For pathways and functional processes, you can go through Kegg, Metacore, Genecode web portals to identified miRNA regulated pathways. For data representation, its upto you in which manner you need to show your data? or It depends on what type of data you generated from your studies?
Hope it works!!!
@ Tanisha Singh
Thank you for the reply... but these portals does not perform analysis for multiple miRNA (all up or all down regulated) and also no filtering option. One miRNA (miR-122-3p) has 796 transcripts sites. How to streamline the analysis for 25 miRNAs
miRPATH http://snf-515788.vm.okeanos.grnet.gr/
You can add multiple miRNA i.e. either up regulated or the down regulated based on your analysis, and look at pathways that are significantly effected by that combination of miRNA.
You can also specify which target prediction software/ algorithm: TargetScan, DIANA-microT-CDS or DIANA-TarBase.
You may also select how you want to display results.
Please see help page: http://snf-515788.vm.okeanos.grnet.gr/index.php?r=site/help&topic=mirpath
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biomolecules
- Nucleic Acids
- RNA
- Small RNA
- microRNA