I am currently using WPI's UPUMP microinjector to inject zebrafish embryos, and I am having problems with my needle leaking when it comes into contact with the solution the embryos are in. For context, I am pulling my needles (thin wall, no filament, 1 mm OD, 0.75 mm ID) and I break the tip off using forceps. I have tried glass with and without filament and breaking the needle tip at different diameters, but no matter what I do, the needle tip leaks.
The machine does allow the compensation pressure to be adjusted, but again, it does not matter what I have it on (I have tried almost every possible pressure; even 0 PSI still causes leakage). Any suggestions? Has anyone used this equipment before and encountered this issue?
Hi, I am not familiarized with that specific microinjector, but in our lab we used to have the same problem with the Narisigue IM-300 microinjection. We fixed it by cleaning inside the injection holder were you insert the neddle and changing the rubber inside the holder cap. Hope it helps.
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