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I've generated an enhancer knock-out in mice, which I then did qPCR of the gene it potentially regulates and seen it is 25% down-regulated in the homozygous knock-out. So I decided to do RNA Seq...
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Hi all, These days, I tried to move an insert originally in the TA cloning vector (pGEM-T Easy, Promega) to my target plasmid vector. I used SacII and NotI to cut TA cloning vector, ran gel and...
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