Microglial specific marker for cells in culture, non-rabbit host?

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Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

How and to what extent does routine functional assessment in primary care settings influence exercise habits among older adults?

Regular assessments can help practitioners and clients identify areas that need improvement, such as balance, strength, endurance, and flexibility. This knowledge can motivate the older adult to...

20 April 2024 9,861 1 View

Improved housing for seniors from the LGBQT+ community & the Roma and Traveller Communities?

Hi there, I have already put a question for a policy response. My team and I have been tasked with producing a Rapid Evidence Assessment (REA) on how housing can be improved for our senior...

15 March 2024 7,984 0 View

Can anyone give me examples of policies that have provided good housing options to senior citizens?

Hi there, I am studying for a part-time master's degree in public policy at DCU, Ireland. My group has been asked to prepare A rapid policy assesment on housing options for senior citizens. I...

01 March 2024 948 1 View

Have Citizens Assembly Worked a Succesfull Structure or Platform for Policy Making?

I am doing a policy assignment of 3000 words, due on Monday night on the policy reccomendations of the Irish Citizen's Assembly of Drugs Policy 2023. I would be grateful if anyone has conducted...

07 December 2023 8,704 1 View

Has the decriminalization of illegal drugs in some countries been a complete success?

I have a policy assignment to submit by Monday. I have chose the recent report of the Irish Citizens Assembly on Drugs Policy. www.citizens assembly. I will concentrate on the the top 6...

06 December 2023 7,095 0 View

Can fellow researchers assist me with a critical policy policy analysis -worth 50% of my module mark due in a week?

Dear Researchers. I have an assignment due in a week worth half the module mark. The briefing for the policy assignment is "For this assessment, you are asked to find and read a police report or...

02 December 2023 9,346 0 View

Can fellow researchers give me their opinons on how intesectionality and woke are relevant?

I have a public policy theory assignment on intersectionality and public policy. We are expected before the Tuesday night deadlne to address the connection between intersectionality and woke, as...

25 November 2023 2,723 6 View

Can anyone give me their opinions on intersectionality theory and its implications for public policy?

I would like idead and discussion on the contribution made by intersectionality s to the advancing ways in which intersectionality theory could extend efforts to elucidate public policy outcomes...

19 November 2023 8,003 10 View

COXI as a mitochondrial marker: content or function?

I am performing immunofluorescence stainings of mouse tissue for mtCOX1 (subunit of complex 4 of the ETC). I'm seeing conflicting results about whether COX1 can be used to measure mitochondrial...

09 August 2023 8,105 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Steffen Storck

We are using the Anti-Iba1 antibody (ab5076) from abcam a lot. It is raised in goat and works well in brain sections.

Let me know if you have further questions.

Steffen

Mohammad Aqdas

P2Y12 is an exclusive marker for microglia. You can also try CX3CR1, CD11b, CD45 intermediate.

Good luck.