Under my personal criterion, it is better to separate both activities, in our laboratory Vero cell cultures are maintained in an area and laminar flow hood separated from the room where we infect those cells with intracellular parasites mainly, the laminar flow hood is also different . In your case one would think Staphylococcus aureus strains are extracellular, but that vision has changed. Attach a reference that might help you.

Curr Protoc Microbiol. 2007 Feb;Chapter 9:Unit 9C.4. doi: 10.1002/9780471729259.mc09c04s4.

Tissue culture assays used to analyze invasion by Staphylococcus aureus.

Cheung AL1, Bayles KW.

Author information

Abstract

Many successful pathogens have developed the ability to adhere to and invade animal tissues. Recent experimental evidence suggests that S. aureus, generally perceived as an extracellular pathogen, can also invade and, in some cases, multiply within host cells. As a proxy to infections in animal hosts, the study of S. aureus interactions with tissue culture cells has become an important research tool in many aspects of bacterial pathogenesis. In this unit, we describe two cell culture models, including bovine mammary epithelial cells and human umbilical vein endothelial cells, that investigators have used to study the interactions of S. aureus with host cells.

PMID:

18770623

DOI:

10.1002/9780471729259.mc09c04s4

Reference 2

Thank you so much for the resources, José. Much appreciated!

Never ever incubate mammalian cells and bacterial cultures in the same room. You will end up with a mess earlier or later.